Thermo Fisher Scientific NEDD-9/CASL/HEF1 Recombinant Rabbit Monoclonal Antibody (BLR248L)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunoprecipitation (IP)

6 µL/mg of lysate

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293F

Class

Recombinant Monoclonal

Type

Antibody

Clone

BLR248L

Immunogen

Peptide representing a region between residue 150 and 200 of Human Enhancer of filamentation 1 (aka NEDD-9). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HEF1,uniProtId:Q14511-1,ncbiNodeId:9606,antigenRange:150-200,antigenLength:834,antigenImageFileName:A700-248_HEF1_Q14511-1_Rabbit.svg,antigenImageFileNamePDP:A700-248_HEF1_Q14511-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

BBS, pH 8.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

HEF-1 is a multifunctional protein involved in integrin-based signaling that affects cell motility, growth, apoptosis and oncogenic transformation. The Cas family of docking proteins have been the subject of intense research because of their role in cell motility, growth, apoptosis and oncogenic transformation. These proteins are substrates of focal adhesion kinase (FAK) and the Src family of tyrosine kinases two active targets for drug development. HEF1 protein production increases levels of mRNA transcripts that encode proteins associated with motility, cell transformation and invasiveness, including several metalloproteinases, MLCK, p160ROCK and ErbBi. HEF1 overproduction also mediates apoptosis in epithelial-derived cell lines, including MCF7 and HeLa cells. Recent clinical studies at another institution have found that overexpression of BCAR1 (p130Cas), a related protein, is associated with tamoxifen resistance. This highlights the importance of studying the role of this family of proteins in cancer prognosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.