Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), Brilliant Ultra Violet 661

Applications

• Intracellular staining followed by flow cytometric analysis

Tested Dilution: ≤ 0.25 µg/test

Species Reactivity

Mouse, Rat

Product Specifications

Available Formats

• Alexa Fluor 488
• APC
• Biotin
• Brilliant UV 395, 615, 737, 805
• Brilliant Violet 421, 650, 711, 786
• eFluor 450, 506, 660
• FITC, PE, PE-Cyanine7, PE-eFluor 610, PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The eBio17B7 antibody reacts with mouse and rat IL-17A without cross-reactivity to IL-17F. IL-17A is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in autoimmune and inflammatory diseases such as rheumatoid arthritis, asthma, multiple sclerosis, and psoriasis.

This antibody is validated for intracellular staining followed by flow cytometric analysis of mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended use ≤ 0.25 µg per test (100 µL final volume).

Instrument Compatibility:
Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and requires a cytometer equipped with a 355 nm ultraviolet laser.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation Guidance:
Samples can be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark.

Excitation / Emission / Laser:
350 nm / 660 nm / Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in autoimmune conditions. It forms a 32 kDa disulfide-linked homodimer and signals through IL-17R. IL-17A enhances ICAM-1 expression and stimulates secretion of IL-6, IL-8, G-CSF, and PGE2. It regulates NF-κB and MAPK pathways and contributes to nitric oxide production.

For Research Use Only. Not for use in diagnostic procedures.

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