Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), Brilliant Ultra Violet 661, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 496
• Brilliant UV 615
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 650
• eFluor 450
• eFluor 506
• eFluor 660
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

Description:
The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFNγ), a 17 kDa factor produced by activated T and NK cells with antiviral and antiparasitic activity. IFNγ synergizes with TNFα to inhibit cell proliferation and modulate immune responses through high-affinity receptors.

Applications Reported:
Suitable for intracellular staining followed by flow cytometric analysis.

Applications Tested:
Pre-diluted and validated for intracellular staining of human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00). Recommended use: 5 µL (0.5 µg) per test (100 µL final volume).

Fluorochrome Information:
Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is designed for cytometers with a 355 nm UV laser.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect from light to avoid photo-induced oxidation.

Fixation:
Samples may be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54).

Excitation / Emission / Laser:
350 nm / 660 nm / Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates and is used under license. Powered by Sirigen™.

Target Information

IFN gamma (Interferon gamma, Type II interferon):
A macrophage activation factor produced by T-lymphocytes and NK cells in response to antigens and cytokines. It functions as a dimeric glycoprotein (~45 kDa) with antiviral, antiproliferative, and immunomodulatory activities. IFNγ induces MHC I/II expression, activates macrophages, and enhances immunoglobulin secretion.

Human IFNγ shows species specificity, with no cross-reactivity to mouse IFNγ. It is regulated by IL-2, FGF basic, and EGF, and downregulated by vitamin D3 or DMN. Mutations in the IFNγ gene are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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