Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), Brilliant Ultra Violet 661, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 650
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation available

Product Specific Information

The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4+ T cells. TNF alpha has cytolytic activity against various tumor cells and plays an important role in immune regulation. It exists as dimers, trimers, and as a 26 kDa membrane-bound form.

• Applications Reported: Intracellular staining followed by flow cytometric analysis.
• Applications Tested: Pre-diluted and tested using Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00).
  Recommended usage is 5 µL (0.5 µg) per test (100 µL final volume).
  Cell number may vary from 10⁵ to 10⁸ cells/test.
• Fluorochrome Information: Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is designed for UV laser (355 nm) cytometers.
• Buffer Recommendation: When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.
• Light Sensitivity: Protect vial and samples from light.
• Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54).

Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine of the TNF superfamily, mainly secreted by macrophages. It binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR, regulating immune cells, proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha shows a single 17 kDa band under non-reducing conditions. Knockout studies suggest neuroprotective functions and tumor necrosis activity. It is implicated in autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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