Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES3-9D7), PE-Cyanine7, eBioscience
인간 IL-10을 인식하는 JES3-9D7 클론의 Rat IgG1 단일클론 항체로, PE-Cyanine7 형광표지. Flow cytometry용으로 최적화되어 있으며, 광민감성 주의 필요. 4°C 암조건 보관, Affinity chromatography 정제.
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Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES3-9D7), PE-Cyanine7, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry | 5 µL (0.06 µg)/test | View 11 publications |
| ELISA | - | View 1 publication |
| Functional Assay | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rhesus monkey |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | JES3-9D7 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573523 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Brilliant UV 395
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 711
- eFluor 450
- eFluor 660
- Functional Grade
- PE
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
Description:
The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).
Applications Reported:
Reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested:
Pre-titrated and tested for intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Refer to BestProtocols®: Protocol A for intracellular proteins. Use 5 µL (0.06 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Light Sensitivity:
Tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in dark at 4°C with minimal impact on brightness and FRET efficiency.
Excitation / Emission / Laser:
488–561 nm / 775 nm; Blue, Green, Yellow-Green lasers.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
IL-10 encodes a cytokine primarily produced by monocytes, with some lymphocyte production. It regulates immune responses and inflammation by down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It enhances B cell survival, proliferation, and antibody production, blocks NF-kappa B activity, and regulates the JAK-STAT pathway. Knockout studies suggest IL-10 is critical for intestinal immune regulation. Mutations are associated with increased susceptibility to HIV-1 infection and rheumatoid arthritis. Related diseases include Graft-Versus-Host Disease and HIV-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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