Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio13A), PE-Cyanine7, eBioscience
Mouse IL-13을 인식하는 eBio13A 클론 단일클론 항체로, PE-Cyanine7 형광체 결합 제품입니다. Flow cytometry에 최적화되어 있으며, 0.5 µg/test로 사용 가능합니다. 알레르기 염증 및 면역 조절 연구에 적합하며, 빛에 민감하므로 암조건 보관이 필요합니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio13A), PE-Cyanine7, eBioscience
Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio13A), PE-Cyanine7, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 45 publications |
| ELISA | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio13A |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573529 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC-eFluor 780
- Brilliant UV 737
- Brilliant UV 805
- eFluor 450
- eFluor 660
- Functional Grade
- PE
- PE-eFluor 610
- PerCP-eFluor 710
- Custom conjugation available
Product Specific Information
Description:
The eBio13A antibody reacts with mouse IL-13, a cytokine mainly produced by Th2 cells and antigen-primed CD8 T cells. IL-13 is involved in allergic inflammation, parasitic clearance, and cancer models, showing either inhibitory or stimulatory activity depending on tumor type. In humans, IL-13 contributes to isotype switching in B cells and down-modulates macrophage activity by reducing pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12).
Applications Reported:
This antibody is reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested:
Tested by intracellular staining of stimulated mouse splenocytes. Recommended usage ≤ 0.5 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Titrate for optimal performance.
Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark, with minimal impact on brightness and FRET efficiency.
Excitation / Emission / Laser:
488–561 nm excitation, 775 nm emission; compatible with Blue, Green, and Yellow-Green lasers.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. It regulates inflammatory and immune responses, down-regulates macrophage activity, and inhibits production of pro-inflammatory cytokines and chemokines. IL-13 is involved in allergen-induced asthma independent of IgE and eosinophils, regulates B cell maturation and differentiation, up-regulates CD23 and MHC class II, and promotes IgE isotype switching. Structurally, IL-13 resembles IL-3, IL-4, IL-5, and GM-CSF. The IL-13 gene is located on chromosome 5q within a cytokine gene cluster near IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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