Thermo Fisher Scientific FOXP3 Polyclonal Antibody, DyLight 650

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

-

Flow Cytometry (Flow)

1:8,000

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A peptide correpsonding to amino acids 43-100 of mouse FOXP3 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:FOXP3,uniProtId:Q99JB6-1,ncbiNodeId:10090,antigenRange:43-100,antigenLength:429,antigenImageFileName:PA5-22773_FOXP3_Q99JB6-1_Rabbit.svg,antigenImageFileNamePDP:PA5-22773_FOXP3_Q99JB6-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

DyLight™ 650 DyLight™ 650 DyLight™ 650

Excitation/Emission Max

651/673 nm View spectra

Form

Liquid

Concentration

0.83 mg/mL

Purification

Protein G

Storage buffer

50mM sodium borate

Contains

no preservative

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11157452

Product Specific Information

This antibody has shown reactivity against Ms (CD4+CD25+ T cells), does not react with Hu Jurkat, Ms CD4+CD25- T cell lysates.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.