Thermo Fisher Scientific HIF1A Polyclonal Antibody, DyLight 650
HIF1A 단백질을 인식하는 DyLight 650 결합 토끼 폴리클로날 항체로, WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합합니다. 저산소 조건에서 HIF-1α 발현 증가를 검출하며, 651/673 nm 형광 특성을 가집니다. 연구용 전용 제품입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–10 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | 5–10 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Flow Cytometry (Flow) | Assay-Dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Fusion protein including residues 530–825 of mouse HIF-1 alpha protein |
| Conjugate | DyLight™ 650 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C, store in the dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11155425 |
Product Specific Information
This antibody detects upregulation of HIF-1 alpha in hypoxic samples.
Target Information
HIF1-alpha (HIF1A) is a subunit of HIF1, a transcription factor found in mammalian cells under reduced oxygen tension. HIF-1 is a heterodimer composed of alpha and beta subunits, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors.
HIF1 regulates the adaptive response to hypoxia by activating transcription of more than 40 genes involved in oxygen delivery and metabolic adaptation, including erythropoietin, glucose transporters, glycolytic enzymes, and VEGF.
HIF1-alpha also modulates hypoxia-mediated apoptosis, cell proliferation, and tumor angiogenesis. It interacts with p53 and p300/CBP complexes, influencing hypoxia-responsive gene expression.
In humans, HIF1A is located on chromosome 14q and is implicated in diseases such as ischemia, cancer, pulmonary hypertension, and chronic obstructive pulmonary disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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