Thermo Fisher Scientific FOXP3 Polyclonal Antibody, DyLight 550
FOXP3 단백질을 인식하는 DyLight 550 결합 폴리클로날 항체로, WB, IHC, ICC, Flow Cytometry 등 다양한 응용 가능. 토끼 IgG 기반이며 Bovine, Human, Mouse, Rat 반응성. 556/570 nm 형광 특성으로 Treg 세포 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Flow Cytometry (Flow) | 1:8,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide corresponding to amino acids 43–100 of mouse FOXP3 |
| Conjugate | DyLight™ 550 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Liquid |
| Concentration | 0.9 mg/mL |
| Purification | Protein G |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152275 |
Product Specific Information
This antibody has shown reactivity against mouse CD4+CD25+ T cells, and does not react with human Jurkat or mouse CD4+CD25− T cell lysates.
Target Information
FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals and essential for normal immune homeostasis.
FOXP3 is 381 amino acids long, stably and constitutively expressed at high levels in CD25+CD4+ regulatory T cells, low levels in CD4+CD25− cells, and absent in CD4−CD8+ T cells.
Defects in FOXP3 cause the scurfy phenotype in mice and IPEX syndrome in humans. Transcript variants encoding different isoforms have been identified. In human breast and colon cancer cells, FOXP3 expression is regulated by p53 in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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