Thermo Fisher Scientific EEA1 Recombinant Rabbit Monoclonal Antibody (ARC1250)
EEA1 단백질을 표적하는 재조합 토끼 단클론 항체로, Western blot 및 ELISA에 적합. HEK293 세포에서 발현되며 높은 특이성과 재현성을 제공. 액상 형태로 공급되며 -20°C에서 보관. 연구용으로만 사용 가능.
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Thermo Fisher Scientific EEA1 Recombinant Rabbit Monoclonal Antibody (ARC1250)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat, Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1250 |
| Immunogen | Synthetic peptide corresponding to amino acids 1–100 of human EEA1 (UniProt ID: Q15075) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.4 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849529 |
Product Specific Information
Immunogen sequence:
MLRRILQRTP GRVGSQGSDL DSSATPINTV DVNNESSSEG FICPQCMKSL GSADELFKHY EAVHDAGNDS GHGGESNLAL KRDDVTLLRQ EVQDLQASLK
Target Information
Early endosomal antigen 1 (EEA1) is a 162 kDa membrane-bound protein specific to early endosomes and essential for fusion between early endocytic vesicles. These endosomes mediate redistribution of extracellular compounds.
EEA1 contains zinc-finger-like domains in both N- and C-terminal regions, similar to nucleic acid-binding proteins. The C-terminal zinc-finger domain is conserved across various intracellular trafficking proteins and is critical for EEA1 localization.
Membrane association of EEA1 depends on phosphatidyl 3-kinase activity; inhibitors cause dissociation from early endosomes.
Usage Note
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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