Thermo Fisher Scientific RANBP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

-

Immunocytochemistry (ICC/IF)

2 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) A A A K D T H E D H D T S T E N T(18) C of human RanBP1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RANBP1,uniProtId:P43487-1,ncbiNodeId:9606,antigenRange:1-18,antigenLength:201,antigenImageFileName:PA1-080_RANBP1_P43487-1_Rabbit.svg,antigenImageFileNamePDP:PA1-080_RANBP1_P43487-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325804

Product Specific Information

PA1-080 detects Ran binding protein 1 (RanBP1) from human and mouse cells.

PA1-080 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~30 kDa protein representing RanBP1 from HeLa cell extract.

The PA1-080 immunogen is a synthetic peptide corresponding to residues M(1) A A A K D T H E D H D T S T E N T(18) C of human RanBP1. The PA1-080 immunizing peptide (Cat. # PEP-128) is available for use in neutralization and control experiments.

Target Information

Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.