Thermo Fisher Scientific Ku80 Polyclonal Antibody
Western blot, IHC, IP 등 다양한 응용에 적합한 Rabbit Polyclonal Ku80 항체. 인간 Ku80 단백질(150–200 aa) 영역을 면역원으로 사용. 액상 형태, 0.2 mg/mL 농도, 4°C 보관. DNA 복구 연구용으로 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 150 and 200 of human Lupus Ku autoantigen protein p86 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein, also known as ATP-dependent DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase and functions with the DNA ligase IV–XRCC4 complex in the repair of DNA double-strand breaks by non-homologous end joining and in V(D)J recombination. This gene complements the Chinese hamster xrs-6 mutant defective in DNA repair and recombination. A rare microsatellite polymorphism in this gene is associated with cancer and varying radiosensitivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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