Thermo Fisher Scientific Brachyury Monoclonal Antibody (X1AO2), eBioscience
인간 Brachyury 단백질을 인식하는 단클론 항체로, Western blot, IHC(P), ICC/IF 등 다양한 응용에 적합합니다. 마우스 IgG2b 아이소타입이며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. 배아 발생 및 중배엽 분화 연구에 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-Dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | X1AO2 |
| Immunogen | E.coli-derived fragment of human Brachyury (amino acids 237–348) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865439 |
Product Specific Information
Description:
The monoclonal antibody X1AO2 recognizes human Brachyury, a 47 kDa nuclear transcription factor encoded by the T gene. It binds to specific DNA regions near the palindromic sequence TCACACCT (T-site) through the N-terminal T-box domain. Brachyury regulates midline development and mesoderm formation during embryogenesis and serves as a key mesoderm marker distinguishing embryonic stem cell differentiation into mesenchymal lineages.
Mutations in the T gene are associated with neural tube defects and chordomas. Elevated Brachyury expression is observed in advanced lung tumors and various tumor cell lines.
Applications Reported:
- Western blotting
- Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
- Immunocytochemistry
- Microscopy
Applications Tested:
- IHC (P): ≤ 5 µg/mL (using low pH antigen retrieval)
- ICC/IF: ≤ 5 µg/mL (fixed and permeabilized human cells)
Antibody titration is recommended for optimal performance.
Purity: >90%, determined by SDS-PAGE
Aggregation: <10%, determined by HPLC
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Brachyury is an embryonic nuclear transcription factor that binds to the palindromic T-site via its N-terminal T-box region. It regulates transcription of genes essential for mesoderm formation and differentiation and is localized to notochord-derived cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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