Thermo Fisher Scientific Collagen X Monoclonal Antibody (X53), eBioscience
Collagen Type X 단백질을 인식하는 X53 단클론 항체로, 인간, 마우스 등 다양한 종에 반응합니다. IHC, ELISA, ICC 등 다양한 응용 가능하며, 고순도(>90%)로 정제되었습니다. 뼈 성장 및 연골 석회화 연구에 적합한 연구용 항체입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 18 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL | View 1 publication |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | View 1 publication |
| ELISA | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Guinea pig, Human, Mouse, Pig, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | X53 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865441 |
Additional Formats:
Product Specific Information
The monoclonal antibody X53 recognizes Collagen Type X from human, mouse, porcine, guinea pig, and rat.
X53 does not cross-react with collagen types I, II, III, IV, or VI.
Collagen Type X is a homotrimeric short-chain collagen with limited expression in normal adult tissues. It is mainly expressed during bone growth and development, localized to the peri- and extracellular matrix of hypertrophic chondrocytes during endochondral ossification, and plays a role in cartilage mineralization. Elevated expression has been observed in various tumor types, particularly in the vasculature.
Mutations in the COL10A1 gene encoding Collagen Type X are associated with Schmid type metaphyseal chondrodysplasia.
Applications Reported:
Microscopy, immunohistochemical staining, ELISA, and immunocytochemistry.
Applications Tested:
Tested by immunohistochemistry on formalin-fixed paraffin-embedded human tissue using low pH antigen retrieval. Recommended concentration ≤10 µg/mL. Optimize dilution for specific assays.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Type X collagen is produced by hypertrophic chondrocytes and localized to presumptive mineralization zones of hyaline cartilage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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