Thermo Fisher Scientific Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), eBioscience
Alpha-Smooth Muscle Actin(α-SMA)을 인식하는 1A4 클론 단일클론 항체로, 사람, 생쥐, 랫드에 반응합니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 사용됩니다. 평활근세포 표지 및 종양 진단 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100 | 9 |
| Immunohistochemistry (IHC) | - | 38 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1 µg/mL | 1 |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | 21 |
| Flow Cytometry (Flow) | Assay-dependent | 5 |
| Immunoprecipitation (IP) | Assay-dependent | - |
| In vitro Assay (IV) | - | 1 |
| Miscellaneous PubMed (Misc) | - | 1 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Chicken, Human, Mouse, Pig, Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865421 |
Available Conjugated Formats
- Alexa Fluor 488 (Cat. No. 53-9760-82)
- eFluor 570 (Cat. No. 41-9760-82)
- eFluor 660 (Cat. No. 50-9760-82)
- Custom conjugation available upon request
Product Specific Information
Description:
The monoclonal antibody 1A4 recognizes human, mouse, and rat alpha-smooth muscle actin (α-SMA), a 42 kDa cytoskeletal protein expressed in smooth muscle cells, myofibroblasts, and myoepithelial cells. It is useful for identifying leiomyomas, leiomyosarcomas, and pleomorphic adenomas, and serves as a prognostic marker for basal cell carcinoma.
Applications Reported:
The 1A4 antibody has been reported for use in:
- Intracellular staining followed by flow cytometric analysis
- Immunoprecipitation
- Western blotting
- Immunohistochemistry (frozen and paraffin-embedded tissues)
- Microscopy and immunocytochemistry
Applications Tested:
- IHC (FFPE human tissue): ≤1 µg/mL
- ICC (formaldehyde or methanol-fixed cells): 1 µg/mL
- WB (reduced rat and mouse intestine): ≤5 µg/mL
Antibody concentration should be optimized for each assay.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Smooth Muscle Actin (α-SMA) is a member of the actin protein family involved in cell structure, motility, and contraction.
- Alpha actin: major component of the contractile apparatus
- Beta and gamma actins: involved in cell motility regulation
Smooth muscle actin is found in vessel walls, gut wall, myometrium, and myoepithelial cells of breast and salivary glands.
Mutations in the smooth muscle actin gene are linked to familial thoracic aortic aneurysm type 6.
Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells and appears in fibroblastic stress fibers during wound healing and fibrocontractive diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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