Thermo Fisher Scientific PUF60 Polyclonal Antibody
Thermo Fisher Scientific의 PUF60 폴리클로날 항체는 Human PUF60 단백질(1–50 aa)에 특이적으로 반응하며, WB, IHC, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 4°C에서 보관합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific PUF60 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 and 50 of Human Poly-U Binding Splicing Factor 60KDa |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with Orangutan.
Target Information
PUF60 encodes a Ro RNP-binding protein that interacts with Ro RNPs, potentially representing a gain of function for these complexes.
It forms a ternary complex with FUSE and FUSE-binding protein, repressing c-myc reporter activity via FUSE.
PUF60 targets transcription factor IIH and inhibits activated transcription.
This gene is implicated in xeroderma pigmentosum disorder and has two alternatively spliced transcript variants encoding different isoforms.
Evidence suggests multiple polyadenylation sites for this gene.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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