
Thermo Fisher Scientific PUF60 Polyclonal Antibody
Thermo Fisher Scientific의 PUF60 폴리클로날 항체는 Human 및 Mouse에 반응하며 Western blot, IHC, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 400 and 450 of Human Poly-U Binding Splicing Factor 60KDa |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Rat, Bovine, and Orangutan
Target Information
PUF60 encodes a Ro RNP-binding protein that interacts with Ro RNPs, potentially conferring a gain of function. It forms a ternary complex with FUSE and FUSE-binding protein, repressing a c-myc reporter via FUSE. It also targets transcription factor IIH and inhibits activated transcription. This gene is implicated in xeroderma pigmentosum disorder. Two alternatively spliced transcript variants encode different isoforms, and multiple polyadenylation sites are reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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