Thermo Fisher Scientific Phospho-PKM2 (Ser148) Polyclonal Antibody
PKM2 단백질의 인산화된 Tyr148 잔기를 특이적으로 인식하는 rabbit polyclonal antibody. Western blot 및 IHC(P) 실험에 적합. Human, Mouse, Rat 반응성. 고순도, 1 mg/mL 농도, -20°C 보관.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human PKM (Accession P14618), corresponding to amino acid residues around phosphorylated Tyr148 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816927 |
Product Specific Information
Antibody detects endogenous levels of PKM2 only when phosphorylated at Tyr148.
Target Information
PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 interacts with thyroid hormone and may mediate metabolic effects induced by thyroid hormones. It also binds Opa protein, a bacterial outer membrane protein involved in gonococcal adherence and invasion of human cells, suggesting a role in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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