Thermo Fisher Scientific Phospho-BRAF (Ser729) Polyclonal Antibody
BRAF 단백질의 Ser729 인산화 상태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat 반응성. 1 mg/mL 농도의 액상 형태로 제공되며, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human BRAF (Accession P15056), corresponding to amino acid residues around phosphorylated Ser729 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816929 |
Product Specific Information
This antibody detects endogenous levels of B-Raf only when phosphorylated at Ser729.
Target Information
BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer and is thought to play a role in the postsynaptic responses of hippocampal neurons. This protein regulates the MAP kinase/ERKs signaling pathway, affecting cell division, differentiation, and secretion. Mutations in B-Raf have been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and lung adenocarcinoma. A pseudogene for B-Raf is located on chromosome X.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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