Thermo Fisher Scientific Pokemon (LRF) Monoclonal Antibody (13E9), eFluor 660, eBioscience
인간 LRF(POKEMON) 단백질을 인식하는 13E9 단클론 항체로 eFluor 660 형광체가 결합되어 있습니다. 면역조직화학, 세포면역염색 등에 사용 가능하며, 651/668 nm 파장에서 적색 레이저로 검출됩니다. 4℃ 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | - |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | View 1 publication |
| Flow Cytometry (Flow) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 13E9 |
| Conjugate | eFluor™ 660 |
| Excitation/Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574200 |
Product Specific Information
This monoclonal antibody reacts with human Leukemia/lymphoma-related factor (LRF), also known as POKEMON, FBI-1, and Zbtb7a. LRF is a 65-kDa transcriptional repressor expressed in hematopoietic stem cells, T/B lymphocytes, macrophages, and erythroid cells. It is highly expressed in non-Hodgkin’s lymphoma and diffuse large B-cell lymphoma, implicating its role in oncogenesis.
LRF regulates B cell lineage commitment by opposing Notch signaling and is critical for erythroid differentiation by inhibiting Bim-mediated apoptosis. It also interacts with Bcl-6 in germinal center B cells.
Applications Reported:
- Immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections
- Immunocytochemistry
Applications Tested:
Tested by IHC on human FFPE tissue using high pH antigen retrieval at ≤5 µg/mL. Recommended to titrate for optimal performance.
Fluorochrome Information:
eFluor™ 660 replaces Alexa Fluor™ 647.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
LRF plays a key role in directing early lymphoid progenitors toward B cell lineage by repressing T-cell instructive Notch signals. It represses transcription of the CDKN2A gene and inhibits E2F1-dependent CDKN2A transactivation. Binds to consensus sequence 5'-[GA][CA]GACCCCCCCCC-3'.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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