Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (29A1.4), eFluor 660, eBioscience
마우스 NKp46(CD335)을 인식하는 단클론 항체로, NK 세포 표지 및 분석에 적합합니다. eFluor 660 형광체 결합으로 레드 레이저(633 nm)에서 검출 가능하며, Flow Cytometry에 최적화되어 있습니다. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (29A1.4), eFluor 660, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 22 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 29A1.4 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10598664 |
Alternative Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 711
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation available
Product Specific Information
The monoclonal antibody 29A1.4 recognizes mouse NKp46 (CD335), a member of the natural cytotoxicity receptor (NCR) family. CD335 is a glycoprotein with two Ig-like domains and a short cytoplasmic tail, expressed specifically on NK cells (including immature NK cells, DX5– CD3–). This allows discrimination between NKT cells and NK cells (NKp46+, CD3–).
Staining with 29A1.4 is not strain-specific and has been validated on C57Bl/6, SJL, CBA/CA, and BALB/C strains. NKp46 plays a role in NK cell-mediated lysis of tumor and pathogen-infected cells.
- The 29A1.4 antibody activates NK cells in vitro but does not deplete NK cells in vivo.
- Reported application: Flow cytometric analysis.
- Tested application: Flow cytometric analysis of mouse splenocytes (≤1 µg per test, 100 µL final volume).
- Recommended to titrate for optimal assay performance.
eFluor® 660 replaces Alexa Fluor® 647, emits at 659 nm, and is excited with the red laser (633 nm).
Ensure instrument compatibility for detection.
Excitation: 633–647 nm
Emission: 668 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
NKp46 (CD335, NCR1) is a cytotoxicity-activating receptor that enhances the efficiency of activated NK cells in mediating tumor cell lysis. It consists of two Ig-like domains related to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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