Thermo Fisher Scientific Jagged 2 Monoclonal Antibody (HMJ2-1), eFluor 660, eBioscience

Applications

• Flow Cytometry (Flow): Tested at 1 µg/test

Product Specifications

Additional Formats

• PE
• Request custom conjugation

Product Specific Information

This HMJ2-1 monoclonal antibody reacts with mouse Jagged-2, a Notch receptor ligand. Upon binding to Notch receptors (Notch 1–4), Jagged-2 undergoes proteolytic cleavage by ADAM-family metalloproteases and γ-secretase, releasing an intracellular fragment with uncertain biological function. Jagged-2 is expressed by thymic lymphoid and stromal cells, macrophages, and dendritic cells in the spleen.

Note: Staining of endogenous splenic dendritic (CD8⁺CD11c⁺) cells was confirmed in C57BL/6 mice but not Balb/c. Other strains not tested. The antibody cross-reacts with rat and may have blocking function.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤1 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize titration for best performance.

Fluorochrome: eFluor® 660 (replacement for Alexa Fluor® 647)

• Excitation: 633–647 nm
• Emission: 668 nm
• Laser: Red Laser

Filtration: 0.2 µm post-manufacturing filtered

Target Information

The Notch signaling pathway is essential for embryonic development and cell fate determination. JAG2 acts as a ligand activating Notch and related receptors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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