Thermo Fisher Scientific RELM beta Polyclonal Antibody, PeproTech
RELM beta 단백질을 표적으로 하는 토끼 폴리클로날 항체로, 마우스 시료에 반응합니다. Western blot, ELISA 등 다양한 응용에 적합하며 항원 친화 크로마토그래피로 정제되었습니다. 동결건조 형태로 제공되며 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific RELM beta Polyclonal Antibody, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | 4 publications |
| Immunohistochemistry (IHC) | – | 2 publications |
| Flow Cytometry (Flow) | – | 2 publications |
| ELISA | 0.5–2.0 µg/mL | 2 publications |
| In vitro Assay (IV) | – | 2 publications |
| Miscellaneous PubMed (Misc) | – | 1 publication |
| Immunostaining (IS) | – | 9 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine RELM beta |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929855 |
Product Specific Information
AA Sequence of Recombinant Protein:
MQCSFESLVD QRIKEALSRQ EPKTISCTSV TSSGRLASCP AGMVVTGCAC GYGCGSWDIR NGNTCHCQCS VMDWASARCC RMA
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine RELMbeta. Anti-Murine RELMbeta-specific antibody was purified by affinity chromatography employing an immobilized Murine RELMbeta matrix.
Sandwich ELISA:
To detect Murine RELMbeta by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. When used with PeproTech Biotinylated Anti-Murine RELMbeta (500-P215Bt) as detection antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Murine RELMbeta.
Western Blot:
For Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. When combined with compatible secondary reagents, detection limit for Recombinant Murine RELMbeta is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
Adipose tissue serves as an energy reserve and secretes various physiologically important proteins including LPL, leptin, Acrp30, perilipin, and resistin. Resistin (also known as Adipocyte Secreted Factor, ADSF, or FIZZ3) is a cytokine secreted by adipocytes. It plays a role in insulin resistance and glucose metabolism, with elevated serum resistin levels observed in obese, diabetic mice. Expression of resistin requires C/EBP alpha and is influenced by coactivators CBP and p300 with histone acetyltransferase activity. PPAR gamma and RXR ligands have been shown to downregulate resistin expression in adipocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(이미지 파일명: 500-P215-100UG_RELM_beta_Q99P86-1_Rabbit_PDP.jpeg)
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