Thermo Fisher Scientific RELM beta Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 Rabbit Polyclonal Anti-Murine RELMβ 항체로, Western blot과 ELISA에 최적화됨. 항원 친화 크로마토그래피로 정제되었으며, -20°C에서 보관. PeproTech의 캡처 항체와 함께 사용 시 높은 민감도의 샌드위치 ELISA 검출 가능.
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Applications 및 권장 희석 배수
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.25–1.0 µg/mL | 3 publications |
| In vitro Assay (IV) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
| Immunostaining (IS) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived, 18.0 kDa Recombinant Murine RELMβ |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
AA Sequence of recombinant protein:
MQCSFESLVD QRIKEALSRQ EPKTISCTSV TSSGRLASCP AGMVVTGCAC GYGCGSWDIR NGNTCHCQCS VMDWASARCC RMA
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine RELMβ. The antibody was purified by affinity chromatography and subsequently biotinylated.
Sandwich ELISA:
- Recommended concentration: 0.25–1.0 µg/mL (100 µL/well)
- In conjunction with PeproTech Polyclonal Anti-Murine RELMβ (500-P215) as a capture antibody
- Detection limit: 0.2–0.4 ng/well of Recombinant Murine RELMβ
Western Blot:
- Recommended concentration: 0.1–0.2 µg/mL
- Detection limit: 1.5–3.0 ng/lane under reducing or non-reducing conditions
- Compatible with appropriate secondary reagents
Packaging:
500-P215BT-1MG is provided as 2 × 500 µg vials.
Target Information
Adipose tissue serves as a tightly regulated energy reserve in animals, secreting various physiologically important proteins such as lipoprotein lipase (LPL), leptin, Acrp30, perilipin, and resistin.
Resistin (also known as Adipocyte Secreted Factor, ADSF, or FIZZ3) is a cytokine secreted by adipocytes and has been implicated in insulin resistance and type 2 diabetes.
Studies indicate that obese, diabetic mice have elevated serum resistin levels, which antagonize insulin action.
Resistin gene expression requires C/EBPα, with coactivators CBP and p300 facilitating histone acetylation at the resistin promoter, enhancing transcriptional activation.
PPARγ and RXR ligands have been shown to downregulate resistin expression in adipocytes.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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