Thermo Fisher Scientific FTO Recombinant Rabbit Monoclonal Antibody (JM98-30)
FTO 단백질을 인식하는 토끼 유래 재조합 단일클론 항체로, 높은 특이성과 민감도를 제공. WB, IHC(P), ICC/IF 등 다양한 응용 가능. HEK293 세포에서 발현된 항체로 로트 간 일관성 우수. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:200 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM98-30 |
| Immunogen | Synthetic peptide within Human FTO aa 1–50 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810061 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
FTO is a member of the non-heme dioxygenase (Fe(II)- and 2-oxoglutarate-dependent dioxygenases) superfamily. Amino acid conservation suggests that both 2-oxoglutarate and iron are essential for FTO function.
FTO may influence gene activity related to metabolism and fat storage, potentially affecting obesity risk.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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