Thermo Fisher Scientific GCLM Recombinant Rabbit Monoclonal Antibody (JM39-61)
GCLM 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC 등 다양한 응용에 사용 가능. 높은 특이성과 일관성을 제공하며, 단기간 4°C, 장기간 -20°C 보관 권장. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC-F) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM39-61 |
| Immunogen | Recombinant protein within Human GCLM aa 225–274 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2800360 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed in HEK293 cells. Individual clones are screened to select the best candidates for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Gamma-glutamylcysteine synthetase (gamma-GCS) is the rate-limiting enzyme for glutathione (GSH) synthesis, a vital antioxidant in mammalian cells. Gamma-GCS is a heterodimer composed of a heavy catalytic subunit and a light regulatory subunit, responsive to inflammation, phenolic antioxidants, heat shock, oxidants, and cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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