Thermo Fisher Scientific SMAD1 Monoclonal Antibody (OTI1C2), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:4,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

The SMAD1 gene encodes a member of the SMAD family, which are signal transducers and transcriptional modulators involved in multiple signaling pathways.
SMAD1 mediates signals from bone morphogenetic proteins (BMPs), regulating cell growth, apoptosis, morphogenesis, development, and immune responses.
Upon activation by BMP receptor kinase, phosphorylated SMAD1 forms a complex with SMAD4 to regulate transcription.
SMAD1 is targeted by SMURF1 and SMURF2 E3 ubiquitin ligases for ubiquitination and proteasome-mediated degradation.
Alternatively spliced transcript variants encoding the same protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.