Thermo Fisher Scientific MYC Epitope Tag Polyclonal Antibody, DyLight 649
MYC epitope tag 인식용 DyLight 649 결합 polyclonal antibody로 Western blot, ICC/IF, ELISA에 적합. Rabbit IgG 기반, 높은 감도와 특이성 제공. 형광 특성(651/673 nm)으로 단백질 발현 검출에 활용 가능. 연구용 전용 제품.
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Thermo Fisher Scientific MYC Epitope Tag Polyclonal Antibody, DyLight 649
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified from rabbit serum immunized with MYC epitope tag peptide (E-Q-K-L-I-S-E-E-D-L) conjugated to KLH. Sequence corresponds to amino acids 410–419 of human c-Myc. |
| Conjugate | DyLight™ 649 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot and freeze at -20°C or below. Avoid repeated freeze/thaw cycles. Centrifuge if solution is not clear after standing. Stable for several weeks at 4°C as undiluted liquid; dilute only prior to immediate use.
This affinity-purified antibody specifically recognizes the MYC epitope tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy-termini of target proteins expressed in transfected or transformed cells. Suitable for detecting MYC-tagged proteins in various assays.
Target Information
Myc is a proto-oncogene encoding a nuclear phosphoprotein involved in cell cycle progression, apoptosis, and cellular transformation. The protein forms a heterodimer with MAX, binds to the E-box DNA consensus sequence, and regulates transcription of target genes. Gene amplification is common in human cancers, and translocations are linked to Burkitt lymphoma and multiple myeloma. Translation can initiate from both a non-AUG (CUG) and an AUG start site, producing two isoforms with distinct N-termini.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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