Thermo Fisher Scientific V5 Epitope Tag Polyclonal Antibody, DyLight 649
V5 epitope 태그 검출용 DyLight 649 결합 다클론 항체로, Western blot, ICC/IF, ELISA에 적합. Rabbit IgG 기반, 높은 특이성과 감도 제공. 형광 특성(651/673 nm)을 가지며 연구용 단백질 발현 확인에 활용 가능.
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Thermo Fisher Scientific V5 Epitope Tag Polyclonal Antibody, DyLight 649
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified antibody from rabbit serum immunized with synthetic peptide aa 95–108 (G-K-P-I-P-N-P-L-L-G-L-D-S-T) of V protein conjugated to KLH using maleimide |
| Conjugate | DyLight™ 649 |
| Excitation/Emission Max | 651/673 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid; dilute only prior to immediate use.
This affinity purified antibody is directed against the V5 epitope tag and is useful for detecting its presence in overexpressed proteins across various assays.
Recognizes the V5 epitope tag sequence (Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr) fused to either amino- or carboxy-termini of target proteins in transfected or transformed cells.
Target Information
Epitope tags enable localization and study of gene products, protein topology, and identification of associated or poorly immunogenic proteins when specific antibodies are unavailable.
The V5 epitope tag originates from the paramyxovirus SV5 P and V proteins, typically using the full 14-amino-acid sequence (GKPIPNPLLGLDST) or a shorter 9-amino-acid form (IPNPLLGLD).
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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