Thermo Fisher Scientific Phospho-AMPK alpha-1 (Ser486) Polyclonal Antibody
Phospho-AMPK alpha-1 (Ser486) 폴리클로날 항체로 인간, 마우스, 랫트 반응성. Western blot 및 IHC에 적합하며, 높은 순도(>95%)와 특이성 보유. 액상 형태로 1 mg/mL 농도, 항원 친화 크로마토그래피로 정제됨. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | View publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human AMPK alpha1 around the phosphorylation site of Serine 486 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553615 |
Product Specific Information
- Detects endogenous protein at a molecular weight of 63 kDa.
- Does not cross-react with phosphorylated AMPK alpha-2 or other related proteins.
- Purity >95% by SDS-PAGE.
Target Information
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the γ phosphate of ATP, onto an acceptor amino acid in a substrate protein. Through this mechanism, they mediate signal transduction in eukaryotic cells, regulating metabolism, transcription, cell cycle progression, cytoskeletal rearrangement, cell movement, apoptosis, and differentiation.
With over 500 gene products, the protein kinase family is one of the largest families in eukaryotes, classified into 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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