Thermo Fisher Scientific Phospho-ATF2 (Thr73) Polyclonal Antibody
ATF2 단백질의 Thr73 인산화 부위를 인식하는 Rabbit Polyclonal Antibody로, IHC 및 IP에 사용 가능. Human, Mouse, Rat 반응성. 고순도(>95%) 항원 친화 정제. PBS/glycerol buffer에 보관하며 연구용으로 적합.
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Thermo Fisher Scientific Phospho-ATF2 (Thr73) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human ATF2 around the phosphorylation site of Threonine 73 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553620 |
Product Specific Information
- Detects endogenous protein at a molecular weight of 55–75 kDa.
- Purity is >95% by SDS-PAGE.
Target Information
ATF-2 (also called CRE-BP1) is a transcription factor that binds to both AP-1 and CRE DNA response elements and belongs to the ATF/CREB family of leucine zipper proteins.
It interacts with viral oncoproteins and cellular tumor suppressors and is a target of SAPK/JNK and p38 MAP kinase signaling pathways.
Cellular stress such as genotoxic agents, inflammatory cytokines, and UV irradiation stimulate ATF-2 transcriptional activity through phosphorylation at Thr69 and Thr71.
Both SAPK and p38 MAPK phosphorylate ATF-2 at these sites in vitro and in transfected cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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