Thermo Fisher Scientific Phospho-Bcl-2 (Thr56) Polyclonal Antibody
인간, 마우스, 랫트 시료에서 Bcl-2 Thr56 인산화 단백질을 검출하는 폴리클로날 항체. IHC, ICC/IF, IP에 적합하며, 항원 친화 크로마토그래피로 정제됨. 고순도(>95%)로 1 mg/mL 농도, PBS/glycerol 저장용액에 보관. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human Bcl-2 around the phosphorylation site of Threonine 56 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553622 |
Product Specific Information
- Detects endogenous Bcl-2 protein at approximately 26 kDa.
- Purity >95% by SDS-PAGE.
Target Information
BCL-2 is a key regulator of apoptosis that can inhibit or promote cell death. Members of the BCL-2 family form dimers to modulate apoptosis. For example, Bag1 forms a heterodimer with BCL-2 to enhance its anti-apoptotic effect. Bax and Bak are involved in cytochrome c release from mitochondria, initiating apoptosis. Constitutive expression of BCL-2, such as translocation to the Ig heavy chain locus, is associated with follicular lymphoma. Overexpression of BCL-2 has been linked to cancers including B-cell lymphoma and prostate cancer. Two transcript variants of BCL-2 differ at their C-terminal ends.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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