Thermo Fisher Scientific Phospho-ASK1 (Ser83) Polyclonal Antibody
Phospho-ASK1 (Ser83) 단백질을 특이적으로 인식하는 Rabbit Polyclonal Antibody. Western blot 및 IHC(P) 검증 완료. 고순도 항원 친화 크로마토그래피 정제. PBS/glycerol buffer에 보관하며 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human ASK1 around the phosphorylation site of Serine 83 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553617 |
Product Specific Information
- Detects endogenous protein at a molecular weight of 155 kDa.
- Purity >95% by SDS-PAGE.
Target Information
MAP3K5 (ASK1) is a serine/threonine kinase abundantly expressed in human heart and pancreas. It is activated by various extracellular stimuli including cytokines, growth factors, and environmental stresses. ASK1 activates MAPKKs MKK4 and MKK6, which in turn activate JNK and p38 MAP kinases. It is activated by TNFR and Fas via TRAF family proteins and Daxx. Overexpression induces apoptosis, while inactive ASK1 inhibits TNF-α-induced apoptosis. ASK1 is expressed in various human and mouse tissues.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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