Thermo Fisher Scientific c-Myc Chimeric Recombinant Rat Monoclonal Antibody (9E10)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Epitope sequence: EQKLISEEDL, located in amino acids 410–419 of human c-Myc protein.

Target Information

The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays a crucial role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.

Phosphorylation of c-Myc at Thr58/Ser62 by enzymes such as glycogen synthase kinase 3, cyclin-dependent kinase, ERK2, and JNK is associated with cell proliferation and regulation. c-Myc is essential for vasculogenesis and angiogenesis during tumor development.

The c-myc oncogene (p62 c-myc) regulates normal cellular proliferation and differentiation, while deregulated expression induces apoptosis in multiple cell types. Antibodies against c-Myc epitopes recognize overexpressed proteins containing Myc epitope tags fused to amino- or carboxy-termini of target proteins.

Amplification of the c-Myc gene occurs in various human tumors including lung, breast, and colon carcinomas. The N-Myc gene is amplified in neuroblastomas. Chromosomal translocation of the c-myc locus on chromosome 8 to immunoglobulin loci on chromosomes 14, 2, or 22 is characteristic of Burkitt’s lymphoma and other B-cell lymphoproliferative disorders.

Aberrant expression of c-Myc has been observed in multiple tumor types such as colorectal, gastric, hepatic, mammary, ovarian, endometrial, pulmonary, prostatic, thyroidal, and hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.