Thermo Fisher Scientific c-Myc Chimeric Recombinant Rat Monoclonal Antibody (9E10)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Epitope sequence: EQKLISEEDL, located in amino acids 410–419 of human c-Myc protein.

Target Information

The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is frequently activated in tumor cells and plays critical roles in cellular proliferation, differentiation, apoptosis, and cell cycle progression.

Phosphorylation of c-Myc at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK is associated with regulation of proliferation and cell cycle. c-Myc is essential for vasculogenesis and angiogenesis during tumor development.

The c-Myc oncogene (p62 c-Myc) regulates normal cellular growth and differentiation; deregulated expression induces apoptosis in various cell types. Antibodies against c-Myc epitopes recognize Myc-tagged fusion proteins at either N- or C-termini.

Amplification of c-Myc is observed in multiple human cancers, including lung, breast, and colon carcinomas. N-Myc amplification occurs in neuroblastomas. Translocation of the c-Myc locus (chromosome 8) to immunoglobulin loci on chromosomes 14, 2, or 22 is characteristic of Burkitt’s lymphoma and other B-cell proliferative disorders.

Aberrant c-Myc expression is found in tumors of colorectal, gastric, hepatic, mammary, ovarian, pulmonary, prostatic, thyroid, and hematopoietic origins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.