Thermo Fisher Scientific TRAP1 Monoclonal Antibody (3H4-2H6), FITC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3H4-2H6

Immunogen

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Conjugate

FITC FITC FITC

View additional formats

• APC
• PerCP
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine

Contains

no preservative

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931763

Product Specific Information

1 µg/mL of MA5-45309 was sufficient for detection of Trap-1/HSP75 in 20 µg of Human A431 lysate by ECL immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.|Detects approximately 75kDa.

Target Information

The 90 kDa heat shock protein (hsp90) family of molecular chaperones is a highly conserved family of proteins that play an important physiological role. Hsp90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of hsp90 is the tumor necrosis factor receptor-associated protein (TRAP1). Like hsp90, TRAP1 is found to be associated with numerous proteins involved in diverse actions. Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting presequence strongly implicates TRAP1 as a mitochondrial matrix protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.