Thermo Fisher Scientific SERPINH1 Monoclonal Antibody (1C4-1A6), FITC
SERPINH1(HSP47) 단백질을 인식하는 FITC 결합 단클론 항체로, 인간 시료에 반응합니다. Western blot과 면역세포염색(ICC/IF)에 적합하며, 47 kDa 단백질 검출에 유용합니다. 단백질 G 정제, 보존제 무첨가, 4°C 암소 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1C4-1A6 |
| Immunogen | Human HSP47, full length (SERPINH1, UniProt ID: P50454-1, antigen range 1–418) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931756 |
Additional Formats
- Unconjugated (MA5-27711)
- APC (MA5-45301)
- PE (MA5-45304)
- PerCP (MA5-45303)
- Request custom conjugation
Product Specific Information
1 µg/mL of MA5-45302 was sufficient for detection of HSP47 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects a 47 kDa protein.
Target Information
SERPINH1 is a member of the serpin superfamily of serine proteinase inhibitors, and its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen, acting as a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to SERPINH1 have been found in patients with rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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