Thermo Fisher Scientific CENPA Monoclonal Antibody (5A7-2E11), PE
PE 형광으로 표지된 인간 CENPA 단클론 항체로, Western blot 및 면역세포염색에 적합합니다. 마우스 IgG1 아이소타입이며, 565/576 nm에서 여기/방출 특성을 가집니다. 단백질 G로 정제된 액상 형태로 제공되며, 연구용으로만 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5A7-2E11 |
| Immunogen | Synthetic peptide corresponding to a portion of human CENP-A |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931754 |
Additional Formats
- Unconjugated (MA5-27710)
- APC (MA5-45297)
- FITC (MA5-45298)
- PerCP (MA5-45299)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45300 was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 18 kDa. Recognizes Human CENP-A.
Target Information
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes.
CENPA encodes a centromere protein containing a histone H3-related histone fold domain required for targeting to the centromere.
CENPA is proposed to be part of a modified nucleosome structure, replacing one or both copies of conventional histone H3 in the (H3-H4)₂ tetrameric core of the nucleosome particle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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