Thermo Fisher Scientific NEFM Monoclonal Antibody (NF-09)
신경세포 내 중간 필라멘트 단백질 NEFM을 인식하는 단클론 항체로, Western blot 및 면역염색에 적합합니다. 인산화 및 비인산화 형태 모두 반응하며, 다양한 포유류 종에 교차 반응성을 가집니다. 단백질 A로 정제된 액상 항체로 4°C 보관합니다.
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Thermo Fisher Scientific NEFM Monoclonal Antibody (NF-09)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mammal, Mouse, Pig, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NF-09 |
| Immunogen | Pellet of pig brain cold stable proteins after depolymerization of microtubules |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1077187 |
Product Specific Information
This antibody reacts with both phosphorylated and non-phosphorylated forms of medium neurofilament protein (160 kDa intracellular antigen) of various species.
Immunocytochemistry protocol:
Carnoy’s fixative 2 × 3 min, blocking with 1% glycine + 0.2% gelatin for 10 min.
Target Information
Neurofilaments (NFs) are intermediate filaments expressed almost exclusively in neuronal cells, predominantly in large axons. They consist of three polypeptide chains — NF-M (medium), NF-H (high), and NF-L (light) — which share structural similarity but differ in terminal sequences.
NF-M’s tail domain mediates interactions critical for axonal size and cytoskeletal architecture, regulated by phosphorylation and O-GlcNAcylation.
Antibodies against neurofilament subunits serve as robust neuronal markers due to their abundance, neuron-specific expression, and biochemical stability.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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