Thermo Fisher Scientific HLA-G Monoclonal Antibody (MEM-G/1)
HLA-G 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot 및 Immunohistochemistry에 사용 가능. 비결합형 액상 형태이며, Protein A로 정제됨. 인간 시료에 반응하며 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | – | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:60–1:100 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEM-G/1 |
| Immunogen | Recombinant human HLA-G denaturated heavy chain |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1076732 |
Additional Formats:
Product Specific Information
This antibody reacts with an extracellular epitope of denaturated HLA-G heavy chain.
HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and is expressed on the surface of trophoblast cells.
Immunohistochemistry (Paraffin): Incubation for 1 hour at room temperature (RT).
Target Information
HLA-G is part of the HLA class I heavy chain paralogues. It forms a heterodimer with beta-2 microglobulin, with the heavy chain anchored in the membrane.
HLA-G is expressed on fetal-derived placental cells. The heavy chain (~45 kDa) gene contains 8 exons:
- Exon 1: leader peptide
- Exons 2–3: α1 and α2 domains (peptide-binding)
- Exon 4: α3 domain
- Exon 5: transmembrane region
- Exon 6: cytoplasmic tail
HLA and MHC antibodies are crucial in Immunopeptidomics, aiding neoantigen identification and characterization via LC-MS/MS analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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