Thermo Fisher Scientific GAPDHS Monoclonal Antibody (Hs-8)
GAPDHS 단백질을 인식하는 마우스 단클론 항체로, Western blot, ICC/IF, Flow cytometry에 적합합니다. 사람 및 돼지 시료에 반응하며, 순도 95% 이상입니다. 액상 형태로 제공되며 4°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 3–12 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Pig |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Hs-8 |
| Immunogen | Freshly ejaculated human sperms |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | TBS, pH 8 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_2222271 |
Additional Formats
Product Specific Information
This antibody reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.
For ICC staining, membrane permeabilization with acetone is essential.
Purity is >95% by SDS-PAGE.
Target Information
Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme involved in glycolysis, catalyzing the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate.
GAPDH is widely expressed and often used as a housekeeping control in mRNA and protein expression studies.
It interacts with various proteins, including amyloid precursor protein and Huntingtin, and has been implicated in neurodegenerative diseases such as Alzheimer’s and Huntington’s disease.
GAPDH also associates with actin and tubulin and may play a role in apoptosis regulation, translocating to the nucleus during apoptotic processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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