Thermo Fisher Scientific GAPDHS Monoclonal Antibody (Hs-8)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Flow Cytometry (Flow)

3-12 µg/mL

-

Product Specifications

Species Reactivity

Human, Pig

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

Hs-8

Immunogen

Freshly ejaculated human sperms. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GAPDHS,uniProtId:O14556-1,ncbiNodeId:9606,antigenRange:1-408,antigenLength:408,antigenImageFileName:MA1-19235_GAPDHS_O14556-1_House_mouse.svg,antigenImageFileNamePDP:MA1-19235_GAPDHS_O14556-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• FITC
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

TBS, pH 8

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_2222271

Product Specific Information

This antibody reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.

For ICC staining, membrane permeabilization with acetone is essential.

Purity is >95% by SDS-PAGE.

Target Information

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important house keeping functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimers disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntingtons disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.