
Thermo Fisher Scientific Phospho-EEF2K (Thr348) Polyclonal Antibody
Phospho-eEF2K (Thr348) 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. -20°C 보관, 연구용 전용 제품입니다.
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Applications and Tested Dilution
- Western Blot (WB): 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-eEF2K (Thr-348) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Thr-348 in human eEF2K. This sequence is well conserved in rat and mouse eEF2K, and has low homology to other proteins. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942915 |
Product Specific Information
The antibody detects a 105 kDa protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of human recombinant eEF2K autophosphorylated in the presence of Ca²⁺ and Calmodulin. This reactivity is removed after lambda phosphatase treatment.
Target Information
EEF2K (eukaryotic elongation factor 2 kinase) is a highly conserved serine/threonine kinase in the calmodulin signaling pathway. Its activity is upregulated in several cancers, including breast cancer. EEF2K phosphorylates elongation factor EEF2 at a specific site, preventing EEF2 from binding ribosomes and thereby reducing the rate of protein synthesis. Activation can occur through upstream kinases such as AMPK or TRPM7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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