Thermo Fisher Scientific Phospho-EEF2K (Ser445) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-eEF2K (Ser-445) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-445 in human eEF2K. This sequence is well conserved in rat, mouse, and fish eEF2K, and has low homology to other proteins.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942918

Product Specific Information

The antibody was cross-absorbed to unphosphorylated eEF2K (Ser-445) before affinity purification using phospho-eEF2K (Ser-445) peptide (without carrier). This antibody detects a 105 kDa* protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of Jurkat cells treated with Calyculin A, and this reactivity is removed after lambda phosphatase treatment.

Target Information

EEF2K is a highly conserved Serine/Threoning kinase in the calmodulin signaling pathway whose activity is increased in cancers including breast cancer. EEF2K (eukaryotic elongation factor 2 kinase) is a threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Up activation, by a variety of upstream kinases including AMPK or TRPM7, EEF2K phosphorylates the elongation factor EEF2 at a single site. Then, EEF2K renders EEF2 unable to bind ribosomes and inactive - in turn, the rate of protein synthesis is reduced.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.