Thermo Fisher Scientific Phospho-EEF2K (Ser445) Polyclonal Antibody
Phospho-eEF2K (Ser445)를 인식하는 Rabbit Polyclonal Antibody로, Human, Mouse, Rat에 반응합니다. Western blot에 최적화되어 있으며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 -20°C에서 보관하며 연구용으로 사용됩니다.
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Thermo Fisher Scientific Phospho-EEF2K (Ser445) Polyclonal Antibody
Applications
- Western Blot (WB): Tested dilution 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-eEF2K (Ser-445) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-445 in human eEF2K. This sequence is well conserved in rat, mouse, and fish eEF2K, and has low homology to other proteins. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2942918 |
Product Specific Information
The antibody was cross-absorbed to unphosphorylated eEF2K (Ser-445) before affinity purification using phospho-eEF2K (Ser-445) peptide (without carrier). This antibody detects a 105 kDa protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of Jurkat cells treated with Calyculin A, and this reactivity is removed after lambda phosphatase treatment.
Target Information
EEF2K is a highly conserved serine/threonine kinase in the calmodulin signaling pathway whose activity is increased in cancers including breast cancer. EEF2K (eukaryotic elongation factor 2 kinase) regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by upstream kinases such as AMPK or TRPM7, EEF2K phosphorylates elongation factor EEF2 at a single site, rendering EEF2 unable to bind ribosomes and thereby reducing the rate of protein synthesis.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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