Thermo Fisher Scientific Phospho-EEF2K (Ser500) Polyclonal Antibody
Phospho-eEF2K (Ser500) 특이적 인식 항체로 인간, 마우스, 랫트 시료에 반응합니다. Western blot에 최적화되어 있으며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 제공되며 -20°C 보관 시 안정적입니다. 연구용으로만 사용 가능합니다.
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Applications
- Western Blot (WB)
Tested Dilution: 1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-eEF2K (Ser-500) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-500 in human eEF2K. This sequence is well conserved in rat and mouse eEF2K, and has low homology to other proteins. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.75 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2942919 |
Product Specific Information
The antibody detects a 105 kDa protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of human recombinant eEF2K autophosphorylated in the presence of Ca²⁺ and Calmodulin. This reactivity is removed after lambda phosphatase treatment. In addition, the antibody detects eEF2K after calyculin A treatment of mouse C2C12 cells, but not in control cells.
Target Information
EEF2K is a highly conserved serine/threonine kinase in the calmodulin signaling pathway whose activity is increased in cancers including breast cancer. EEF2K (eukaryotic elongation factor 2 kinase) regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by upstream kinases such as AMPK or TRPM7, EEF2K phosphorylates elongation factor EEF2 at a single site, rendering EEF2 unable to bind ribosomes and thus reducing the rate of protein synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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