Thermo Fisher Scientific EEA1 Monoclonal Antibody (GT10811)
EEA1 단백질을 검출하기 위한 Mouse monoclonal antibody로, WB, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat 반응성 확인. 고순도 Protein G 정제, 1 mg/mL 농도, PBS 용액 형태. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT10811 |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Human EEA1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787202 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%).
- Positive Control: 293T, A431, HeLa, HepG2.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Early endosomal antigen 1 (EEA1) is a 162 kDa membrane-bound protein specific to early endosomes and is essential for fusion between early endocytic vesicles.
Early endosomes fuse with endocytic vesicles to redistribute extracellular compounds to alternate destinations.
Zinc-finger-like domains are located in both amino and carboxyl-terminal regions of EEA1.
The carboxyl-terminal zinc-finger-like domain is conserved in other non-nuclear proteins involved in intracellular trafficking and is critical to EEA1 localization.
Membrane association of EEA1 depends on phosphatidyl 3-kinase activity; inhibitors cause EEA1 to dissociate from early endosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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