Thermo Fisher Scientific IL-27 Monoclonal Antibody (234205), FITC

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

10 µL per million cells

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

234205

Immunogen

CHO-derived recombinant mouse IL-27 (aa 29-234 of p28, aa 19-228 of EBI-3) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IL-27,uniProtId:O35228-1,ncbiNodeId:10090,antigenRange:19-228,antigenLength:228,antigenImageFileName:MA5-23551_IL-27_O35228-1_Rat.svg,antigenImageFileNamePDP:MA5-23551_IL-27_O35228-1_Rat_PDP.jpeg,sortOrder:3},{targetFamily:IL-27,uniProtId:Q8K3I6-1,ncbiNodeId:10090,antigenRange:29-234,antigenLength:234,antigenImageFileName:MA5-23551_IL-27_Q8K3I6-1_Rat.svg,antigenImageFileNamePDP:MA5-23551_IL-27_Q8K3I6-1_Rat_PDP.jpeg,sortOrder:4}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

FITC FITC FITC

View additional formats

• PE
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Purification

Protein A/G

Storage buffer

Buffered saline solution with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2610324

Product Specific Information

This antibody recognizes the p28 subunit either as part of a p28/EBI-3 heterodimer or as a free subunit.

Target Information

IL-27 (Interleukin 27) is a cytokine belonging to the IL-12 family, and is a heterodimeric cytokine composed of EBI3 and p28 subunits. IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines. IL-27 promotes CD4+ T cell differentiation to the Th1 lineage by inducing expression of the transcription factor T-bet and up regulating IL-12R beta2, which in turn suppresses Th2 and Th17 differentiation and proliferation. IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. IL-27 acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting IL-27 involvement in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. In humans, the gene is located on the p arm of chromosome 16.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.