Thermo Fisher Scientific Phosphatidylinositol 4,5-bisphosphate Monoclonal Antibody (2C11)
Phosphatidylinositol 4,5-bisphosphate(PIP2)를 인식하는 Monoclonal Antibody(2C11). Western blot, IHC, ICC, ELISA, IP 등에 사용 가능. Liposome 기반 면역원으로 제작되었으며, Mouse IgM 형식의 비결합 항체. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | View 1 publication |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:200 | - |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Chemical |
| Published Species | Chemical |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2C11 |
| Immunogen | Liposomes (prepared in PBS with lipid A, phosphatidylcholine (PC) and cholesterol) containing synthetic di-palmitoyl PtdIns(4,5)P2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Ascites |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_568690 |
Product Specific Information
MA3-500 detects PIP2 from human tissues.
MA3-500 has been successfully used in ELISA, Immunoprecipitation, Immunofluorescence, and Western blotting procedures.
The MA3-500 antigen is liposomes containing synthetic di-palmitoyl PtdIns(4,5)P2.
Target Information
Phosphatidylinositol 4,5-bisphosphate (PIP2) is an important second messenger in numerous signaling processes. Upon extracellular stimulation, phospholipase C cleaves phosphatidylinositol into DAG and PIP2. PIP2 interacts with actin structural proteins such as profilin, gelsolin, and cofilin, regulating actin polymerization. It also regulates vesicular transport by interacting with proteins like ADP-ribosylation factor and phospholipase D, and influences intracellular calcium levels affecting various cellular activities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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