Thermo Fisher Scientific Phosphatidylinositol 4,5-bisphosphate Monoclonal Antibody (2C11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Immunocytochemistry (ICC/IF)

1:200

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Chemical

Published species

Chemical

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

2C11

Immunogen

Liposomes (prepared in PBS with lipid A, phosphatidylcholine (PC) and cholesterol) containing synthetic di-palmitoyl PtdIns(4,5)P2.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_568690

Product Specific Information

MA3-500 detects PIP2 from human tissues.

MA3-500 has been successfully used in ELISA, Immunoprecipitation, Immunofluorescence, and Western blotting procedures.

The MA3-500 antigen is liposomes containing synthetic di-palmitoyl ptdlns(4,5)P2.

Target Information

Phosphatidylinositol 4,5-bisphosphate or PIP2 is an important second messenger for a number of signaling processes. When certain extracellular signals are received, Phosphilipase C cleaves phosphatidylinositol into DAG and PIP2. PIP2 has been shown to interact with actin structural proteins including profiling, gelsolin, and cofilin, and demonstrates regulatory function of actin polymerization. It has also been shown to regulate vesicular transport by interacting with proteins like ADP-ribosylation factor and phospholipase D. PIP2 has also been shown to significantly affect intracellular calcium concentrations, which also regulate a variety of activities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.