Thermo Fisher Scientific p23 Monoclonal Antibody (JJ3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 20 publications 20 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:200-1:2,000

View 4 publications 4 publications

Flow Cytometry (Flow)

1/100

-

Immunoprecipitation (IP)

Assay-dependent

View 5 publications 5 publications

in situ PLA (PLA)

-

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

Assay-dependent

-

Product Specifications

Species Reactivity

Chicken, Guinea pig, Human, Mouse, Rabbit, Rat

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

JJ3

Immunogen

Recombinant human p23 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2175197

Product Specific Information

MA3-414 detects p23 from chicken, guinea pig, human, rat, mouse and rabbit samples.

MA3-414 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 19 or 23 kDa protein representing p23 from rabbit reticulocyte lysate. MA3-414 can be used to immunoprecipitate both free and complexed p23.

The MA3-414 antigen is recombinant human p23.

Target Information

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP70 and HSP90), immunosuppressant binding proteins called immunophilins (the FK506 binding proteins, FKBP52 & FKBP54 and the cyclosporin binding protein, CyP-40) and at least three other proteins termed p23, p60 and p48. p23 along with HSP70, HSP90 and p60, combine with progesterone receptor (PR) as members of a transient intermediate complex. Cloned human p23 encodes a protein of 160 amino acids that is highly conserved between species and shows no homology to previously identified proteins. p23 is a highly acidic phosphoprotein with an aspartic acid-rich C-terminal domain and multiple potential phosphorylation sites. In vitro studies have suggested that p23 binds to HSP90 and is necessary for the binding of HSP90 and CyP-40 to PR. While neither its exact function nor mechanism of action have been identified, p23 appears to be an important factor in PR function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.