Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), Brilliant Violet 421, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.125 µg)/test

Product Specifications

Additional Available Formats

• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Brilliant UV 395, 563, 615, 661, 737, 805
• Brilliant Violet 480, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• NovaFluor Blue 610-70S, 660-120S
• NovaFluor Red 710
• NovaFluor Yellow 610, 660
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610, PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed as either a heterodimer with CD8 beta chain (CD8α/β) or as a homodimer (CD8α/α).
A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and, through association with tyrosine kinase p56lck, plays a role in T-cell development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted; tested on normal human peripheral blood cells at 5 µL/test (0.125 µg).
  • A test = amount of antibody for 100 µL final volume
  • Cell number: 10⁵–10⁸ cells/test (empirically determined)

Brilliant Violet™ 421 (BV421) emits at 423 nm and is suitable for cytometers with a 405 nm violet laser. Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (#SB-4401-42) or
• Brilliant Stain Buffer™ (#00-4409-75)
  to minimize non-specific interactions.

Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family involved in T cell signal transduction, differentiation, and activation.
CD8 is expressed mainly on T cells as a disulfide-linked heterodimer (CD8α/β), acting as a co-receptor for MHC-I molecules, while CD4 serves as a co-receptor for MHC-II.
CD8 also exists as homodimers (CD8α/α) on subsets of T cells, NK cells, monocytes, and dendritic cells.
Binding of MHC-I/peptide complexes to CD8 recruits Lck kinase, leading to cytokine production, motility, and cytotoxic T lymphocyte (CTL) activation.
Activated CTLs play key roles in pathogen and tumor clearance, and their differentiation is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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