Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Brilliant Violet 421, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.125 µg/test
• Publications: View 1 publication

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 480–786
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue/Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436–780
• Request custom conjugation

Product Specific Information

Description:
The M1/70 monoclonal antibody reacts with mouse CD11b (integrin alphaM, 165–170 kDa). CD11b associates with CD18 to form the alphaMbeta2 integrin (Mac-1), binding CD54 (ICAM-1), C3bi, and fibrinogen. It is expressed by macrophages, NK cells, granulocytes, activated lymphocytes, and peritoneal B-1 cells. M1/70 cross-reacts with human CD11b and can detect this antigen on human monocytes, granulocytes, and NK cell subsets. CD11b plays a role in adhesive cell interactions.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse bone marrow cells; ≤0.125 µg/test recommended (100 µL final volume). Optimize empirically for each assay.

Fluorochrome Information:
Brilliant Violet™ 421 (BV421) emits at 423 nm, excited by a 405 nm violet laser. Ensure instrument compatibility.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant UV™, etc.), use:

• Super Bright Complete Staining Buffer (#SB-4401-42)
• or Brilliant Stain Buffer™ (#00-4409-75)
  to minimize non-specific interactions.

Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company. Powered by Sirigen™.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule forming a heterodimer with CD18 (integrin beta-2). The CD11b/CD18 complex (Mac-1, CR3) binds ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X, mediating cell-cell and cell-matrix interactions.

CD11b is expressed on:

• Monocytes/macrophages
• Granulocytes
• Activated lymphocytes
• NK cell subsets
• Dendritic cells
• Microglia

Expression varies by tissue and increases during monocyte maturation. It is commonly used as a microglial marker.

Associated Diseases: Systemic lupus erythematosus 6 and ITGAM-related lupus susceptibility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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